Figure 2. ORFfinder output at NCBI, disclosing all possible open reading frames (ORFs) and their direction within the query DNA
sequence. In addition to the graphic view, details such as ORF coordinates, length, strand, and frame are highlighted in the table
below. By selecting each ORF, it is possible to obtain the translated aminoacid sequence. Immediate BLAST of each ORF may be
executed with the command BLAST ORF.
Figure 3. BLAST output at NCBI, highlighting the similarity scores between the query ORF and the 100 best BLAST hits retrieved
in the database. Clicking over a line displays the alignment between the query sequence and the subject sequence, allowing
identification of differences between the two sequences.