5. Conduct correction routine. This step is included only when
fish chooses the wrong side. No data are recorded during
correction trials. This is a positive reinforcement procedure
to help fish learn correct pattern.
a. Lower gate on the LEFT arm ( i.e., wrong side).
b. Open starting box gate and let fish swim to correct arm.
If necessary, use gentle touches with blunt probe to
encourage fish to enter correct arm.
c. Allow fish to remain in correct arm for 45 seconds.
Then gently place back in starting box.
d. Remove gate on incorrect arm and continue trials.
6. Continue conducting trials until a maximum of 20 trials or
five correct choices within a string of six trials occurs –
whichever comes first.
a. Fish is considered to have learned task of correctly
choosing the RIGHT arm if it has done so for five out
Figure 3. Student photographs of various steps in experiments using T-maze. Photographs provided by Sheridan Schaffer,
Greendale (WI) High School (parental permission provided). (A) Place individual fish into starting block. (B) Allow fish to swim
down raceway. (C) Fish chooses LEFT side, lower gate on RIGHT arm. (D) Vigorously stir for positive punishment.