Immunoassays are used extensively in the biological sciences. This
quick, simple, cost-effective lab exercise introduces students to the
important concepts of specific and nonspecific binding used in a variety of ways in different immunoassays (Tijssen, 1985). The dot-blot
assay is an inexpensive way to test field samples for the presence of
antigen and is a useful vehicle for students to explore product development and marketing in biotechnology.
Thanks to Vickey Tomlinson for enduring many years of student
Graddon, D.J. & Randles, J.W. (1986). Single antibody dot immunoassay:
a simple technique for rapid detection of a plant virus. Journal of
Virological Methods, 13, 63–69.
Guy, P.L. (1988). Pasture ecology of barley yellow dwarf viruses at
Sandford, Tasmania. Plant Pathology, 37, 546–550.
Landry, M.L. (2009). Developments in immunologic assays for respiratory
viruses. Clinics in Laboratory Medicine, 29, 635–647.
McLaughlin, M.R., Larsen, R.C., Trevathan, L.E., Eastman, C.E. & Hewings, A.D.
(1996). Virus diseases of American pasture and forage crops. In Pasture
and Forage Crop Pathology (pp. 289–302). Madison, WI: American
Stave, J.W. (2002). Protein immunoassay methods for detection of biotech
crops: applications, limitations, and practical considerations. Journal of
AOAC International, 85, 780–786.
Tijssen, P. (1985). Practice and Theory of Enzyme Immunoassays.
Amsterdam, The Netherlands: Elsevier.
von Messling, V., Harder, T.C., Moennig, V., Rautenberg, P., Nolte, I. &
Haas, L. (1999). Rapid and sensitive detection of immunoglobulin M
(IgM) and IgCG antibodies against canine distemper virus by a
new recombinant nucleocapsid protein-based enzyme-linked
immunosorbent assay. Journal of Clinical Microbiology, 37,
Wu, M.J., McKay, S., Hegedus, E. & Chin, J. (2012). Proteolytic extraction
enhances specific detection of the novel ‘S’-type low molecular weight
glutenin subunit in wheat by monoclonal antibody. Journal of Cereal
Science, 55, 53–60.
PAUL L. GUY is an Associate Professor in the Department of Botany,
University of Otago, New Zealand; e-mail: email@example.com.
Figure 3. Examples of students’ brochures.