Assessment of Students’ Perceptions of Their Core Competency Gains: SALG Survey Questions (see Figure 1)
Students were asked: Please rate your GAINS in each of the following BIOSCIENCE COMPETENCIES.
[Scale = 1 (no gains), 2 (a little gain), 3 (moderate gain), 4 (good gain), 5 (great gain)]
Mastering the scientific process of investigation (designing and conducting experiments)
Developing basic lab skills (pipetting, making dilutions, following procedures)
Developing analytical and quantitative skills (statistical analysis, graphing)
Understanding the connection between the concepts in biology, chemistry, and mathematics
Assessment of Student Learning Gains: Matched Pretest & Posttest Questions (see Table 2)
Scientific Literature Search: If you had to find a scientific review article about the health effects of different “nutraceutical”
(having nutritional and pharmaceutical properties) compounds in broccoli, how would you go about doing this? Explain in
as much detail as you can.
Simple Dilutions: You are given a stock solution of 1 M glucose and told that you need to prepare 10 mL that has a final
concentration of 10 mM glucose. How do you go about doing this?
Serial Dilutions: By using a standard curve, we will be able to quantify the amount of ATP in our cultures. The manufacturer of our assay kit suggests preparing ATP standards with a final volume of 100 μL and concentrations of 0.01–
1000 nM. Starting with a 1 μM stock solution, what would be the most accurate way to prepare these standards? Show
all your work.
Using a Standard Curve: Biologists often use a spectrophotometer to measure the absorbance of a solution and thereby
determine the concentration of a particular solute in that solution. To do this requires the use of a standard curve in which
different concentrations of known quantities of solute are measured.
a. What is a standard curve and how do you prepare one?
b. Absorbance is a ratio of the level of light passing through a sample over the level of incident light coming from a light
source. As the concentration of solute increases, the absorbance _______________ (choose one: increases/decreases).
c. How can one determine the concentration of the solute in an experimental tissue sample in comparison with the standard
Quantitative Transfer with a Micropipette: Suppose you have to measure out 250 μL of a 10 μM protein solution and transfer
that into a microcentrifuge tube containing 1 mL of water. What is the best way to accurately measure out the 10 μM protein
Assessment of Students’ Perceptions & Affective Outcomes: Course Evaluation Questions (see Figure 2)
For the following questions, response options were “Much Higher,” “Higher,” “Average,” “Lower,” and “Much Lower”:
• The intellectual challenge was . . .
• The amount that I learned was . . .
• The amount of effort expected of students in the course was . . .