( 3) Can pGLO be introduced into eukaryotic microbes such as
yeast? ( 4) How can the intensity of the color of the GFP fluorescence be quantified? Many other kits are currently available from
various suppliers (Bio-Rad Laboratories, Carolina Biological Supply
Company, Ward’s Science, Edvotek) for use by students in AP and
general biology courses. Instructors who have found experiments
they like in these kits might want to develop similar extensions to
those described here.
I thank the students in BIO 318 (Genetics Laboratory) at Creighton
University for trying out many of these extensions of the pGLO
transformation experiment in the fall semesters of 2014 and
2015. I also thank the faculty and staff in the Department of Biology at Creighton University for their hospitality while I was there.
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CHARLES E. DEUTCH is Professor Emeritus in the School of Mathematical
and Natural Sciences, Arizona State University West Campus, Glendale, AZ
85306, USA; email: email@example.com.