collection are currently restricted to on-campus viewing, but the
metadata are discoverable via Google and other web search engines.
Materials & Methods for Fruit Fly &
Carnivorous Plant Projects
Drosophila melanogaster was chosen as the prey because it was readily
accessible and is commonly captured by carnivorous plants in the wild
(Juniper et al., 1989). Both wild-type (WT, Oregon R) and visually
impaired (w1118) Drosophila melanogaster were obtained from Bloomington Stock Center (Indiana). The w1118 flies had a spontaneous
loss of function allele with no functional gene product, or significantly
lower product compared to WT, resulting in visual impairment
(McQuilton et al., 2012). Both WT and w1118 flies were raised in standard fly media (Nutri-Fly BF), incubated at 25°C, and flipped approximately every 2 days. Second-generation flies were used whenever
possible to minimize maternal effects.
Pitcher plants (Nepenthes ventricosa) and sundew plants (Drosera
capensis) were obtained from California Carnivores (https://www.
californiacarnivores.com) and grown in 0.5 L pots under grow lights
on the Augustana University campus in Sioux Falls, South Dakota.
Sundews were planted in a 3:1 peat moss and perlite mixture and
pitcher plants in a 2:1:1 peat moss, perlite, and orchid bark mix.
Sundews were watered twice a week from below using a tray, and
pitcher plants were watered 5 days a week until water ran out the
bottom of the pot.
For the projects exploring the importance of visual cues in the capture process, WT and w1118 flies were exposed to carnivorous plants,
either N. ventricosa or D. capensis, or to artificial traps (Figure 1). Between
30 and 60 flies were placed in 37 L aquariums for 8 hours at ~22°C, with
fluorescent overhead lighting. Total numbers of fly captures were
counted, and this was repeated three to six times. Artificial traps were
constructed using white, black, or red construction paper. Traps con-
sisted of opaque 125 mL flasks and containing standard cornmeal media
(Bloomington recipe) or ~1 g of overripe banana.
During the fall semester of 2017, a Google survey was created and
approved by the Augustana Institutional Review Board. A link to
the Google survey with questions that aligned with several of the
Table 2. Continued
Final Project Key Possible Points
C. Tie back to intro. Discuss results within context of literature 5.0
D. Clear summary/implications 3.0
E. End with strong statement/argument 1.0
F. Voice is consistent – usually third person, past tense 1.0
G. Work is free of grammatical errors 1.0
H. Sentences/word choice are varied, fluent, and effective 1.0
A. Complete list. Proper format, alphabetized, citations in text 5.0
A. Proper headings and page numbers 2.0
B. Is there a strong primary question? 4.0
C. Is there a secondary/follow-up question? 3.0
D. Does the research have novel aspects? 4.0
E. Is there evidence of overall effort/improvement through drafts? 4.0
Figure 1. Experimental setup. Between 30 and 60 fruit flies
were released into a 37 L aquarium with either a carnivorous
plant or an artificial trap.